النشر العلمي

  • Molecular surveillance of antimalarial drug resistance related genes in Plasmodium falciparum isolates from Eritrea.

Abstract

The introduction of artemisinin-based combination therapy has led to extraordinary results in malaria control, however the recent emergence of partial resistance to artemisinin therapy in Southeast Asia jeopardizes these successes. This study aimed at investigating resistance to the antimalarial drugs by evaluating the polymorphisms in the PfK13, Pfcrt and Pfmdr1 genes in Plasmodium falciparum isolates obtained from patients in Eritrea..

published in Acta Trop.

  • Cytokine profiles amongst Sudanese patients with visceral leishmaniasis and malaria co-infections

Background

The immune system plays a critical role in the development of co-infections, promoting or preventing establishment of multiple infections and shaping the outcome of pathogen-host interactions. Its ability to mediate the interplay between visceral leishmaniasis (VL) and malaria has been suggested, but poorly documented. The present study investigated whether concomitant infection with Leishmania donovani complex and Plasmodium falciparum in naturally co-infected patients altered the immunological response elicited by the two pathogens individually.

Results

Circulating levels of interferon (IFN)-γ, interleukin (IL)-2, IL-4, IL-6, IL-10, IL-12p70, IL-13, IL-17A and tumor necrosis factor (TNF) were assessed in sera of patients infected with active VL and/or malaria and healthy individuals from Gedarif State, Sudan. Comparative analysis of cytokine profiles from co- and mono-infected patients highlighted significant differences in the immune response mounted upon co-infection, confirming the ability of L. donovani and P. falciparum to mutually interact at the immunological level. Progressive polarization towards type-1 and pro-inflammatory cytokine patterns characterized the co-infected patients, whose response partly reflected the effect elicited by VL (IFN-γ, TNF) and malaria (IL-2, IL-13), and partly resulted from a synergistic interaction of the two diseases upon each other (IL-17A). Significantly reduced levels of P. falciparum parasitaemia (P <0.01) were detected in the co-infected group as opposed to the malaria-only patients, suggesting either a protective or a non-detrimental effect of the co-infection against P. falciparum infection.

Conclusions

These findings suggest that a new immunological scenario may occur when L. donovani and P. falciparum co-infect the same patient, with potential implications on the course and resolution of these diseases.

published in BMC Immunol. 2014; 15: 16.

  • Diagnostic performance of automated Analyzers versus manual method for WBCs measurement among Population of Wad Madani, Gezira State, Sudan

Abstract: The automated hematology analyzer with complete blood count (CBC) results has replaced the traditional
manual or individual assay methods for hematological parameters and the eye count leukocyte differential as the initial
screening and detection system for hematological abnormalities in modern hospitals and clinics. Automated method for
the estimation of complete blood count is commonly used in routine practice laboratory but many other labs still work on
manual procedure for the abnormal automated results as well as health care workers in laboratory can be optimized by
doing test on manual microscopic procedure as validation technique for automated method. This study carried out to
compare the WBCs by different automated analyzers and manual method. Study carried out in Wad Madani city capital
of Gezira State, central Sudan during the period of July to August 2016. The study included 75 healthy individuals
selected randomly, 31 of them were males and 44 were females, their age ranges from 20 to 50 years who came for
medical examination. Three mls of venous blood were collected from participants in EDTA anticoagulant. The samples
were analyzed using haemocytometer, Sysmex XP-300 and Mindray BC-6800.The results showed that repeatability of
white blood cells by chamber, mindray and sysmex were (5.1707, 5.0293, 4.9560), (5.8680, 5.8573, 5.8507) and (6.0093,
5.9800, 5.9627) ×10³μl respectively, there was no significant different between the repeated result for manual and
mindray but there was significant different by sysmex P.value (0.04). And when we compared the means+ SD of white
blood cells by manual, mindray and sysmex were (5.0520±1.6460), (5.8587±1.7355) and (5.9840±1.7130)× 10³μl
respectively, there was highly significant different by all three method P.value (0.001).Our study concluded that, there
was no significant difference between repeatability of WBCs by manual and Mindray, significant difference between
repeatability of WBCs by Sysmex and application of automated method is easier when there is load of work which takes

  • less time.

published in Scholars Journal of Applied Medical Sciences (SJAMS) ISSN 2320-6691 (Online) Sch. J. App. Med. Sci., 2017; 5(3D):1014-1018

  • Evaluation of Sugar Beet Parameters during Storage
  • Journal of Science and Technology 12 (02) December 2011
  1. ISSN 1605 – 427X
  2. © Sudan University of Science and Technology
  3. www.sustech.edu
  4. Evaluation of Sugar Beet Parameters During Storage
  5. Ebrahim. A. A. Alfaig 1, Kamal Suleiman Hassen2, Awad Elhaj Mohammed3.
  6. 1. Food Science and Technology department, College of Agricultural Studies, Sudan University of
  7. Science & Technology, P.O. Box 71 Khartoum North, Sudan.
  8. 2. Sugar Research Institute, University of Gezira, P.O Box 20, Wad Madani, Sudan.
  9. 3. Sugar Cane Research Center, Sudanese Sugar Company, Elguneid, Sudan.
  10. ABSTRACT: Storability of four sugar beet varieties was tested during the winter
  11. season of 2006 -2007 at Elguneid Sugar Cane Research Center. Sugar beet varieties
  12. used in this study were Juvena, Mashad, Valentina and Brigitta. The storage
  13. conditions were at room, outdoors and underground temperature. The chemical
  14. analysis for samples was carried out for the fresh varieties samples and stated as
  15. control where the rest of the samples were optioned according to the following
  16. schedule: 24 h, 48 h, 72 h, one week, two weeks and one month with average of three
  17. replicates for each test. For the fresh samples of Juvena, Mashad, Valentina and
  18. Brigitta the moisture content was 80.7%, 83.8%, 81.1% and 81.1%, respectively. Ash
  19. content was 0.560%, 0.562%, 0.651% and 0.650%, respectively. Protein content was
  20. 0.210%, 0.226%, 0.202% and 0.190%, respectively. The weight loss % for the tested
  21. varieties after 24 h storage ranged from 5.57% to 8.32% and 5.65% to 6.70% for
  22. room and outdoor samples respectively. The pulp % for fresh tested varieties ranged
  23. from. 2.99% to 3.89%. Purity % for fresh samples was 81.18, 78.59, 82.45 and 79.66
  24. for Juvena, Mashad, Valentina and Brigitta respectively. Reducing sugars% were
  25. 0.066, 0.0083, 0.0116 and 0.005 for the fresh samples of Juvena, Mashad, Valentina
  26. and Brigitta, respectively. The obtained results showed no difference between
  27. varieties, while there were marked differences among the storage methods and
  28. periods. Underground storage scored the best results which were comparable with the
  29. control (fresh).
  30. KEYWORDS: Beta vulgaris, Weight loss, and pulp, purity

published in Journal of Science and Technology

  • Effects of skipping one irrigation at different growth stages on yield and water productivity of some maize (Zeamays L.) cultivars, under heavy clay soil of central Sudan.

published in Gezira Journal of agricultural Sciences, 15 (1): 93 - 107

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