النشر العلمي

  • Molecular Characterization of Bacteria Isolated From Groundnut (Arachis Hypogaea L.) Root Nodules

This study was carried out to characterize three strains of bacteria isolated from groundnut grown in different soils in the Sudan using 16SrRNA gene sequences. The analysis of the sequences revealed that all the three isolates and the reference strains are Klebsiella spp. with similarity ranging from 98% to 99%. However when the sequences were aligned, it was found that there are differences between the different isolated and the reference strains in three positions namely 867, 955 and 964. The differences in the three positions resulted in differences in the translated amino acids of the sequences of the genes also in three positions 289, 319 and 322.

published in International Journal of Life Sciences Research

  • Microbiological Characteristics and Sensory Evaluation of White Cheese Produced by Using Camel Milk and Mixture of Camel and Cow Milk

The objective of the present study was to assess the quality of white cheese produced using raw camel milk, and a mixture of camel milk and cow milk (1:1). Raw milk samples obtained from Wad Medani local markets were subjected to microbiological analysis before production of cheese. Results showed that raw camel and cow milk samples contained 1x104 and 1x106c.f.u/ml total bacterial count and devoid from any coliforms or Staphylococcus aureus cells. Cheeses were prepared by acidification using10% citric acid and by adding lactic acid bacteria (LAB) starter culture (5%). The total bacterial count were 15x106, 9x105 and 11x105 (c.f.u/ml), respectively, while the yeast and moulds counts were 2x103, 6x102 and 8x102 (c.f.u/ml), and the lactic acid bacterial were 1.5x108 3x105and 4x105c.f.u/ml in PCM, MCCM1 and MCCM2, respectively, in cheeses produced from pure camel milk (PCM), mixture of camel milk and cow milk coagulated with acidification (MCCM1) and mixture of camel milk and cow milk coagulated with lactic acid bacterial culture (MCCM2). On the other hand, all samples contained coliform bacterial which ranged between 2.5x105 and 1x106c.f.u/ml, and devoid from any Staphylococcus aureus cells except in the MCCM2 which contained 2x105c.f.u/ml. Mixing of camel milk with cow milk improved the microbiological and sensory quality of the resultant cheese. The sensory evaluation indicated acceptance of all cheeses with preference to those prepared with lactic acid bacteria.

published in Journal of Microbiology Research 2016, 6(1): 8-13

  • The Impact of Individual and Combined Antibiotics against Resistant Bacteria Isolated from Diabetic Foot Infection

Diabetic foot infections are obviously spreading in hospital and facing unique challenge of successful treatment because of wide spread of resistant bacteria to many antibiotics. The clinical use of combination of antibiotics could be one of strategies to combat these resistant bacteria. Thus, basely depends on the microbial etiology and appropriate selective to antibiotics for combination. Using manual E-test method, Minimum inhibitory concentration (MIC) was determined susceptibility to individual antibiotics. Fractional inhibitory concentration index (FICI) was used to determine synergistic effects for combined antibiotics. Gentamicin susceptibility showed 100%, 40%, 80% and 60% of E.coli, Pseudomonas.spp, Staphylococcus aureus, and Proteus spp were sensitive, respectively. Ciprofloxacine showed 90%, 60%, 30% and 80 % resistant to E.coli, Pseudomonas spp, Staphylococcus aureus, and Proteu spp, respectively. Ceftriaxone showed 50%, 50%, 10%, and 80% resistant result against E.coli, Pseudomonas.spp, Staphylococcus aureus, and Proteus spp, respectively. Fractional inhibitory concentration index provided that 20%-40% Pseudomonas strains were synergistic to (gentamicin plus ciprofloxacin) and (gentamicin plus ceftriaxone), respectively. Since 100% of E.coli were antagonism to both combination. However, 80%-90% of Staphylococcus aureus, 70%-80% of Proteus spp showed indifferent effect to (gentamicin plus ciprofloxacin) and (gentamicin plus ceftriaxone), respectively.

published in Journal of Microbiology Research 2016, 6(4): 69-73

  • The Effect of Malting Conditions on the Production of Non alcoholic Sorghum Malt Beverage

The present study aimed to investigate the effect of malting conditions on malt quality to produce non-alcoholic sorghum malt beverage. Feterita sorghum cultivar was used to prepare the sorghum malt. Malting was carried out at two different temperatures of 25°C and 30°C for 4 and 5 days under air-rest and non-aerated conditions. Germination percentage was determined after germinating in an incubator at 30°C for 72 hours. The quality of sorghum malt in terms of malting losses, total loss, diastatic power and α and β amylase was examined. Malted grains germinated for 5 days at 30°C under non-aerated conditions had a greater effect on sorghum malt quality. For germination percentage, the results were 98% and 50% for maximum steep-out moisture. It is concluded that effective use of germination to process sorghum grain for beverage would require a control of the temperature and time. More research is needed in order to understand the adjustments made with different stages of processing of malted beverages.

published in International Journal of Food Science and Nutrition Engineering 2016, 6(4): 81-86

  • دور الباحث النفسي والاجتماعي في المدرسة كما يدركه مديرو المدارس ( دراسة على عينة من مديري ومديرات مدارس الأساس بمحلية ود مدني الكبرى، ولاية الجزيرة، السودان)

هدفت الدراسة الحالية إلى معرفة دور الباحث النفسي والاجتماعي كما يدركه مديرو المدارس بولاية الجزيرة. وقد تكونت عينة الدراسة من (64) مدير ومديرة مدرسة أساس بمحلية ود مدني الكبرى بولاية الجزيرة. وقد قام الباحثان بتصميم أداة لقياس مهام الأخصائي النفسي والاجتماعي الرئيسة والمتمثلة في الكشف المبكر عن المشكلات، الإرشاد النفسي، الاستشارات النفسية والإحالة، وتم تطبيق هذه الأداة على عينة الدراسة الحالية. وقد أسفرت نتائج الدراسة عن فعالية دور الباحث النفسي والاجتماعي داخل مدارس الأساس بمحلية ود مدني الكبرى وفق إدراك مديري مدارس الأساس بمحلية ود مدني الكبرى بولاية الجزيرة، وأسفرت النتائج عن وجود فروق بين الذكور والإناث من أفراد عينة الدراسة في إدراك عمل الباحث النفسي والاجتماعي في مدارس الأساس على محوري  الكشف المبكر عن المشكلات والاستشارات النفسية لصالح الإناث. كذلك أظهرت نتائج الدراسة فروقاً بين مديري مدارس مرحلة الأساس قليلي الخبرة وبين نظرائهم كثيري الخبرة وذلك في إدراكهم لدور الباحث النفسي والاجتماعي في مدارس مرحلة الأساس على جميع محاور  أداة الدراسة وجاءت هذه الفروق لصالح كثيري الخبرة. كما أن نتائج الدراسة لم تكشف عن وجود فروق بين مديري مدارس مرحلة الأساس في إدراك دور الباحث النفسي والاجتماعي في المدرسة وفق متغير المؤهل العلمي (فوق الجامعي، جامعي، أخرى). وفي ضوء هذه النتائج وضع الباحثان عدداً من التوصيات والمقترحات.

published in مجلة جامعة نيلا للعلوم الانسانية

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