النشر العلمي

  • Elhashmi, Y.H.A., Elamin, A. and Hassan, H.E. 2011. Growth and development of muscles, bones and fat of domestic fowl (gallus gallus). Online J. Anim. Feed Res., 1(6): 280-284.

published in OJAFR

  • Relative Growth of Red-Necked Ostrich (Struthio camelus), Guinea Fowl (Numida meleagris) and Domestic Fowl (Gallus gallus)

published in University of Gezira

  • Epidemiological and clinicopathological characteristics of oesophageal cancer in a limited

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published in KESHO 2016

  • In Silico Design of Epitope Based Peptide Vaccine against Virulent Strains of (HN)-Newcastle Disease Virus (NDV) in Poultry Species

Newcastle disease virus (NDV) belongs to the family Paramyxoviridae. It causes Newcastle disease, mainly by the virulent strains of avian paramyxovirus serotype-1 (AMPV-1). This virus represents a huge problem on the world’s economy than any other animal virus, especially in developed countries. ND infection is not obstructed and many studies have attributed the spread of the disease to changes in the genome of the virus and emersion to new strains. We aimed to design a peptide vaccine for NDV particularly for the haemagglutininneuraminidase protein (HN) using computational methods to predict epitopes inducing immune system and can be used later to create a new peptide vaccine could replace conventional vaccines. A total of available 60 virulent strains of HN- NDV were retrieved from NCBI for bioinformatics analysis using Immune Epitope Data Base (IEDB) to predict B and T cells Epitopes. We used human MHC class I and II alleles in this study due to the difficulty to determine MHC B complex alleles in Poultry. Then we docked the best predicted CTL epitopes with B-F alleles (BF2*2101 and BF2*0401). Four CTL cell epitopes namely (548ISNTLFGEF556, 546AEISNTLFG554, 88VALESPLAL96 and 526YTTSTCFKV534) interacted with MHC class (B-F) I alleles and we suggested them to become universal peptides based vaccine against NDV. We found these CTL epitopes to be T helper epitopes also. The overlapping between MHC class I (B-F) and (B-L) II T cell epitopes suggesting the possibility of antigen presentation to immune cells via both MHC class I and II pathways especially the overlapping between 548ISNTLFGEF556 and 546AEISNTLFG554. We considered this study distinctive because no research ever dealt with peptide based vaccine on virulent strains of NDV using in silico approach.

published in International Journal of Multidisciplinary and Current Research

  • Isolation and Biochemical Characterization of Bacteria Isolated from Groundnut (Arachis hypogaea L.) in Sudan

Using of commercial bacteria showed unacceptable results in inoculation trials. This necessitates looking for locally isolated nitrogen fixing bacteria to use as inoculants. This study was done to isolate and select the most efficient bacteria from groundnut (Arachis hypogaea) root nodules. In this study three bacterial strains were isolated from El-Gadarif, El-Gezira and El-Obied soils and pot experiments were done. The study showed that, the field nodules were found distributed on both main and lateral roots of groundnut. The colour of nodules obtained from El-Gadarif soil was pink, while the nodules obtained from El-Gezira and El-Obied soils were of a white colour. All strains were Gram negative, white, circular, viscous, did not absorb Red Congo indicator, produced yellow colour and acidified Yeast Extract Mannitol Agar with bromothymol blue, fast growing, grew in Peptone Glucose Agar medium. All bacterial strains induced nodule formation to groundnut and unable to form nodules in Phaseolus valgaris and Vigna senesis. Inoculation of groundnut by the isolates enhanced groundnut growth. The strain isolated from groundnut nodules in El-Gadarif soil was found the best for groundnut growth promotion. The isolates in the present study showed high symbiotic effectiveness. The study concluded that the strains isolated were fast growing Rhizobia like. Isolation and application of local nitrogen fixing bacteria may resolve the competitiveness and environmental conditions variation and fluctuation problems in the field.

published in International Journal of Scientific Research in Knowledge, 3(11), pp. 0270-276, 2015

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