النشر العلمي

  • MOLECULAR CHARACTERIZATION OF HELICOBACTER PYLORI IN PATIENTS WITH UPPER GASTROINTESTINAL DISORDERS, IN WAD MEDANI, GEZIRA STATE, SUDAN 2017_2018

This cross sectional study aimed for molecular characterization of H.pylori (H.pylori) from gastric biopsy by 16s rRNA from patients with upper gastrointestinal disorders, in Wad Medani city, Gezira State, Sudan. H.pylori is Gram negative curved rod bacterium, its infection distributed worldwide. It is the major cause of gastritis, duodenitis, peptic ulcer, gastric lymphoma, adenocarcinoma and esopharyngeal cancer. This study include 55 patients, 25 males and 30 females complain from gastric disorder attending for Oesophago-gastro duodenoscopy, their age ranged from 17 to 85 years, from September 2017 to April 2018.Two antrum Gastric biopsy Samples were collected for detection of Helicobacter pylori by conventional Polymerase chain reaction (PCR) using primers target 16s rRNA gene and direct Gram stain. Out of 55 samples 24(43,6%) were positive by PCR, out of 50 samples Gram negative curved bacilli were detected in 20 (40%), and DNA sequencing of amplified PCR products of two samples showed substitution in 16s rRNA gene from (A/C) at position 248380 of reference sequence of Helicobacter pylori strains FDAARGOS_300 USA (Accession number CP027404.1). This study concluded that the H. pylori could possibly be considered one of the main causes of gastritis and Duodenitis, also direct detection of H. pylori by Gram stain statistically not differ from PCR.

published in The first young Researcher Conference Genomics and Human Health in Africa- Khartoum and Meroe,2-7 December 2018

  • Molecular Detection of H. Pylori from Gastric Biopsies of Dyspeptic Patients Attending Endoscopy Center, Gezira State, Sudan

Background: H. pylori are Gram negative bacteria cause most common of gastrointestinal tract infections worldwide. H. pylori can lead to serious symptomic or a symptomic illness including ulcers, gastritis, doudenitis, oesophagitis. Serious complications like gastric atrophic, mucosa-associated lymphoid tissue (MALT) lymphoma can occur. H. pylori posses cag A gene which is a virulent factor and marker for the pathogenic strain. This strain associated with greater inflammations and increased the risk of developing both peptic ulcer diseases and gastric carcinoma.

Objective: The study conducted to focus on detection of H. pylori and cag A gene using polymerase chain reaction (PCR) method in biopsy samples from upper gastrointestinal diseases patients in Gezira State, Sudan.

Materials and Methods: Descriptive cross - sectional study was carried out during 2016 – 2019 in Gezira State, Sudan. A total of 102 antrum biopsy samples were collected from adult male and female, their age between (20 – 70 years). Biopsy sample collected by gastroenterologists at Gezira Center for G.I.T. Endoscopy and Laparoscopic Surgery. H. pylori DNA extracted to apply the PCR technique in order to investigate H. pylori infection and cag A gene. In this study PCR for 16s rRNA accepted as gold standard method to identify the H .pylori.

Results: In 102 adult dyspeptic patients (45% male, 55% female, mean of age 46.1 ± 13 years). H. pylori was detected in 53 (51.9%) biopsy samples using PCR 16s rRNA, 22 (41.5%) were positive for cag A. Epigastric pain was a common clinical feature in individuals infected with H. pylori 71 (74%), dyspepsia 28 (29.5%) and vomiting 30 (25.8%). H. pylori infection predominant in gastritis and ulcer patients. Frequency of H. pylori positive cag A was common among in ulcers, gastritis, duodenitis and esophagitis patients. Out of 102 patients 9% reported mass endoscopy finding from them 56% infected with H. pylori when tested by the PCR in those positive H. pylori 80% reported positive cag A.

Conclusion: Frequency of H. pylori infection using 16s rRNA is 53 (51.9%) predominant in ulcers and gastritis patients. Cag A gene highest in gastritis and ulcers patients compare with other diseases, this gene play a role for determination the clinical outcome of H. pylori infection.

published in EUROPEAN ACADEMIC RESEARCH

  • COMPOSITION, ABUNDANCE AND DISTRIBUTION OF AQUATIC WEEDS IN MINOR AND ABUXX CANALS, RAHAD SCHEME, SUDAN

published in الاجتماع العام الخامس عشر لمنتدى الجامعات الإقليمية لرفع القدرات فى الزراعة

  • توجهات المرأة السودانية نحو المشاركة السياسية دراسة إحصائية تحليلية (2016)

published in مجلة الجزيرة للعلوم االاقتصادية والاجتماعية، جامعة الجزيرة، السودان. مجلد(9 ) العدد(1 )2018 م

  • ساحل العاج
ABSTRACT The guts of most insects harbor nonpathogenic microorganisms. Recent work suggests that gut microbiota not only provide nutrients, but also involve in the development and maintenance of the host immune system. However, the complexity, dynamics and types of interactions between the insect hosts and their gut microbiota are far from being well understood. White grub is a polyphagous and nefarious pest of specific significance as it adversely affects the economic status of the farmers. We explored the aerobic and anaerobic gut bacterial flora of larvae of two white grub pests, Anomala dorsalis Fabricius and Lepidiota mansueta Burmeister (Coleoptera : Scarabaeidae) through cultivation-dependent approaches coupled with 16S rRNA gene analysis. Gut bacterial community, consisting of Firmicutes and Proteobacteria were detected from the gut of A.dorsalis and Bacteroidetes and Proteobacteria from the gut of L.mansueta. Furthermore, biochemical characterization of gut bacterial isolates was done by using the Enterobacteriaceae identification kit (HIMEDIA; KB002&KB003) and Phospholipid fatty acid analysis (PLFA) pattern was done to understand the microbiome composition of A. dorsalis. Studies have shown the relative dominance of gram-negative bacteria Biomass content was ranged from 199.28 to in foregut to 351.28 (nmoles/gm) in the fermentation chamber of A.dorsalis. Higher Nitrate reductase was detected from selected gut bacterial isolates incubated under anaerobic conditions. Nitrate reductase activity was significantly higher in Chryseobacterium sp., while, Dysgonomonas termitidis registered the lowest activity of this enzyme. To the best of our knowledge, this is the first attempt to study the midgut microbiota of A. dorsalis from field-collected larvae using "culture-dependent and independent methods". Our results demonstrated that a core microbial community of this insect species. Understanding the functional role of these gut bacterial isolates may pave the way for developing novel pest control strategies. Keywords: whitegrubs, Anomala dorsalis Fabricius and Lepidiota mansueta, microflora

published in AAIS 23 meeting

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